A field experiment was conducted at Zonal Agricultural Research Station, Mandya, Karnataka to study the shift in weed flora of different weed species due to application of pre-sowing, pre-emergence and post-emergence application of herbicides in comparisons with hand weeding and mechanical weeding. The effect of combination of herbicide bensulfuron-methyl + pretilachlor as well as mono-herbicide butachlor  and their post-planting application were evaluated for their bio-efficacy either alone or combination with pre-planting application of non-selective glyphosate application. The treatment comprised of eight different weed management practices were laid out in randomised complete block design with three replications. The results revealed that application of glyphosate (15 days before transplanting of rice 0.75 kg/ha) in combination with bensulfuron methyl + pretilachlor  applied at 5 DAT recorded higher grain yield of 7.02 t/ha and found significantly superior over other treatments due to reduced weed population and biomass of weeds (10.30 g/m2) and higher weed control efficiency (64.97%) when compared with weedy check. There was more than 36% reduction in the grain yield of rice due to competition with weeds in weedy plots. The rice yield and weed control efficiency of herbicide combination was at par with recommended practice of hand weeding twice at 20 and 40 DAT. Sequential herbicide application is promising and effective in control of weeds as compared to single herbicide application in rice-based cropping system