The terminal residues of flufenacet were quantified in soil and wheat grains. Flufenacet was applied at 250 and 300 g/ha on 21 and 35 days after the sowing of wheat at the Research Farm of Punjab Agricultural University, Ludhiana. Matrix solid phase dispersion (MSPD) method was used for the extraction of flufenacet from soil and grain samples. The herbicide residues were quantified using High Performance Liquid Chromatography (HPLC) equipped with UV-Vis detector and were confirmed with gas chromatographic tandem mass spectrometry (GC-MS/MS). The average recoveries of flufenacet extracted from the matrix ranged from 80.9 to 93.0% and 88.0 to 96.2% when quantified using HPLC and GC-MS/MS, respectively with relative standard deviation less than 10%. Both HPLC and GC-MS/MS offer high reproducibility, however GC-MS/MS was more sensitive having limit of detection (LOD) and limit of quantification (LOQ) as 0.001 and 0.003 μg/g, respectively. Terminal residues of flufenacet in the soil and wheat grain samples were below the detectable limit. Thus, the use of flufenacet in wheat under sub-tropical humid conditions could be considered safe.