Over-reliance and continuous use of similar mode of herbicides lead to increase in selection pressure which resulted in evolution of herbicide resistance in weeds. Metsulfuron-methyl is used for the control of broad-leaf weeds in wheat since 1990s. There are reports of failure of control of Rumex dentatus with metsulfuron from farmers’ fields in North-Western India. Pot studies were conducted at Punjab Agricultural University, Ludhiana during winter (Rabi) 2018-19 and 2019-20 to quantify the status and level of herbicide resistance in R. dentatus in Punjab and Haryana. Rumex populations were collected from farmers’ fields in both years. Fifty six and 33 biotypes of R. dentatus from farmers’ fields of Haryana and 6 and 19 biotypes from Punjab were collected in first and second year, respectively. Biotypes were screened using recommended dose of metsulfuron-methyl (5 g/ha) along with unsprayed in pot study. Results revealed that 38 out of 56 biotypes of R. dentatus collected from Haryana in first year were found resistant to metsulfuron whereas only one biotype showed resistance from Punjab. Further, 23 biotypes out of 33 biotypes collected in second year were found resistant from Haryana whereas 14 biotypes out of 19 were found resistant in Punjab. This indicated that R. dentatus has evolved resistance against metsulfuron-methyl in different regions of Punjab and Haryana.