A field experiment was conducted at Bidhan Chandra Krishi Viswavidyalaya, West Bengal, India during 2017-2018 and 2018-19 to study the effect of different herbicide molecules in spring planted sugarcane. Cyperus rotundus, Cynodon dactylon and Alternanthera philoxeroides were dominant weed flora in the sugarcane field. Application of ametryn 1.0 kg/ha at 30 days after planting (DAP) followed by (fb) 2,4-D 1.0 kg/ha at 60 DAP effectively suppressed the grasses, sedges and broad-leaved weeds, improved yield attributes and registered higher cane yield (102.49 t/ha), juice recovery (52.80%) and B:C ratio (2.90) due to better weed control efficiency (71.05%). Next best treatments were atrazine 1.0 kg/ha at 30 DAP fb glyphosate 1.0 kg/ha at 60 DAP; and three hand weeding at 30, 60 and 90 DAP. Application of ametryn 1.0 kg/ha at 30 DAP + 2,4-D 1.0 kg/ha at 60 DAP proved to be a cost-effective option for getting higher cane yield in new alluvial zone of West Bengal.