The present experiment was conducted to study the effect of different mulching materials and herbicide application on weed growth parameters, weed control efficiency (WCE) and corm yield in gladiolus variety ‘Punjab Glance’. The experiment was laid out in a randomized block design with three replications each using polythene mulches of colours, viz. black 25µ, silver on black 25µ, transparent 25µ and white 50 µ along with rice straw mulch at concentrations of 1.0 kg, 1.5 kg and 2.0 kg/m2 respectively. The predominant weeds observed were Oenothera laciniata, Coronopus didymus and Poa annua during November to March. The black and silver on black polythene mulch 25µ along with pendimethalin 0.5 kg/ha reported minimum total weed count, total weed fresh and dry weight. The hand-weeded plot showed maximum WCE (100%) followed by pendimethalin 0.5 kg/ha (99.9%) and similar results were recorded under black and silver on black polythene mulch 25µwith 99.8and 99.5% WCE respectively. The black polythene mulch 25µ best promoted plant growth in terms of corms per plant (1.8) cormels per plant (28.5) and corm weight (61.2 g). It suppressed the weeds efficiently with weed count of only (2.3/m2) from November to March.