Field research was conducted during the winter season of 2020-21 at Central Research Farm of the ICAR-Indian Grassland and Fodder Research Institute, Jhansi, (U. P.) to study the effect of weed management practices on seed yield of berseem (Trifolium alexandrinum L.). The study was conducted in a randomized block design with three replications with a set of seven treatments. The treatment combinations consisted of pre-emergence application (PE) of pendimethalin + imazethapyr and post-emergence application (PoE) of imazethapyr + imazamox, glyphosate followed by (fb) imazethapyr + imazamox, glyphosate fb one hand weeding (HW) along with mechanical stale seedbed, weed free and weedy check. At 30 days after sowing of berseem, application of glyphosate 1.0 kg/ha to kill the existing weed flora before sowing (as chemical stale seedbed) fb one hand weeding and at first and second cut glyphosate 1.0 kg/ ha fb imazethapyr + imazamox 0.07 kg/ha as post emergence were found to be most effective in reducing weed density and biomass. Weed free treatment registered the significantly highest green fodder (28.98 t/ha), straw (3.20 t/ha) and seed yield (545.00 kg/ha) of berseem followed by glyphosate 1.0 kg/ha fb imazethapyr + imazamox 0.07 kg/ha. The highest net returns Rs. 70,597/ha and benefit: cost (2.23) was recorded with glyphosate 1.0 kg/ha fb imazethapyr + imazamox 0.07 kg/ha.