A field experiment was conducted at Agricultural Research Station, Ummedganj, Kota during rainy (Kharif), 2019 to study the comparative efficacy of pre- and post-emergence herbicides in managing weeds and improving productivity and quality of soybean (Glycine max L. Merrill). The experimental field was infested with grassy weeds, broad-leaved weeds and sedges. Maximum number and dry weight of root nodules/plant recorded in hand weeding twice at 20 and 40 days after sowing (DAS) followed by application of acifluorfen-sodium 16.5% + clodinafop-propargyl 8% (pre-mix) 165 + 80 g/ha at 20 DAS, at 50 days after sowing. The lowest dry weight of root nodules/plant was recorded under weedy check. Significantly higher protein content and protein yield were recorded under two hand weeding at 20 and 40 DAS found maximum protein yield followed by application of acifluorfen-sodium 16.5% + clodinafop-propargyl 8% EC (pre-mix) 165 + 80 g/ha at 20 DAS. Oil content in seeds was not significantly influenced by different weed control treatments whereas, oil yield was significantly enhanced by them over weedy check. Oil and protein yield are largely a function of seed yield. Strong positive correlation between oil, protein yield and seed yield support the fact. The data further revealed that two hand weeding at 20 and 40 DAS recorded maximum oil yield followed by application of acifluorfen-sodium 16.5% + clodinafop-propargyl 8% EC (pre-mix) 165 + 80 g/ha at 20 DAS