Experiments were conducted to standardize duration and level of senescence required to enhance translocation of glyphosate in Cyperus rotundus. Senescence was induced by paraquat (500 g/ha) or glyphosate (1.312 kg/ha). Decrease in total chlorophyll and membrane damage was more in herbicide treatments than ethephon. Periodic leaf RWC remained unchanged in control but decreased periodically in ethephon and paraquat. Significantly higher RWC was observed at 72 h in glyphosate than paraquat. Maintenance of membrane integrity and high RWC in glyphosate at 72 h than paraquat facilitated mobility of glyphosate. Total biomass reduction bioassay indicated that senescence induced by glyphosate (1.312 kg/ha) pre-treatment 48 hr followed by (fb) glyphosate (1.312 kg/ha) showed significantly more  efficacy (7%)  than pre-treatment with benzyl adenine  (synthetic cytokinin) fb glyphosate which reduced  efficacy by 9% compared to glyphosate alone.