Lack of glyphosate translocation to the site of action (growing apex) is the limitation for effective control of purple nutsedge (Cyperus rotundus L). Glyphosate is used for weed control in wasteland, zero tillage, directed spray in plantation crops and on quarantine weeds. Senescence of leaf is programmed cell death involving degradation of macromolecules viz. proteins, nucleic acids and lipids and during later stage of this process, essential nutrients are remobilizes to the growing apex. Pre-treatment with ethephon (5000 ppm) induced the leaf senescence and reduced callose accumulation in phloem which enhanced 2,4-D and glyphosate translocation and thus their efficacies (Devendra et al. 1996). Senescence period of 48 h, induced by glyphosate (1.3 kg/ha) enhanced the efficacy more than 24 or 72 h senescence period (Dhanraj et al. 2012). Senescence was found to increase the activity of low affinity (Pht 2,1) phosphate transporter (Daram et al. 1999) and high affinity (PhPT1) phosphate transporter (Chapin and Jones 2009). Glyphosate being phosphate having molecule, compete with phosphate with transporter which led to enhanced phloem mobility.