Assessment of various physico-chemical and biological properties of seed powder of Sida cordifolia was done. This plant grows along roadsides and in open land in tropical climates. Mainly useful plant parts are seeds, leaves and roots. The loose bulk density, tapped bulk density, hausner ratio and angle of repose of sample were found to be 0.31g/cm3±0.1, 0.38 g/cm3± 0.2, 1.22%± 1.6 and 30.96o± 0.7, respectively. Moisture content of sample was found to be 10.00±1.6. Total ash, fat, total protein and non protein nitrogen was 3.80% ± 0.5, 10.56% ± 0.3, 22.00% ± 0.9 and 11.20% ± 0.4, respectively. Thermal stability of seeds was up to 200 oC. Low molecular weight protein bands ranging 10-60 kDa were identified. The demonstration of antimicrobial activity against microbes may be indicative of the presence of broad spectrum antimicrobial compounds. Amide I and amide II bands were two major bands of the infrared spectrum. This powder has very low water absorption capacity.