Terrestrial form of alligatorweed has been reported as a difficult weed to control by the herbicides due to heavy regrowth. No quantitative data is available on regrowth of alligatorweed after herbicides application except visual observations. Hence a comprehensive study was conducted in pot, plot and field conditions to evaluate the effect of three most recommended herbicides in context to superficial control and regrowth. After herbicide application, superficial control of alligator weed was achieved in pot, plot and field conditions but regrowth appeared from the no killed rhizomes. In pot experiment, hundred percent superficial control of alligatorweed at 15 DAA (days after application) was noticed with 2,4-D (2.5 and 3.5 kg/ha) and glyphosate (3.5 and 4.5 kg/ha) and by 20 DAA with metsulfuron-methyl (MSM) at 0.016 and 0.020 kg/ha. In plot experiment, 2,4-D (1.5 kg/ha) and glyphosate (2.0 kg/ha) caused almost 100 percent superficial killing at 10 and 15 DAA, respectively. MSM was most effective at 0.024 kg/ha, however, 0.020 kg/ha was at par with glyphosate (3.0 kg/ha) and 2,4-D (2.0 kg/ha). In plot experiment, little regrowth was noticed in higher doses of glyphosate, 2,4-D and MSM. Repeat application of same herbicides after 90 days of first application revealed no significant difference in regrowth at 30 DAA, however significant difference appeared at 60 and 90 DAA. Effect of MSM (0.020 kg/ha) was at par with higher dose of glyphosate (3.0 kg/ha) on regrowth after repeat application. In naturally infested area, no regrowth appeared in higher doses of glyphosate (3.5 and 4.0 kg/ha) up to 180 DAA, while glyphosate (3.0 kg/ha) and 2,4-D (2.5 kg/ha) were at par with MSM (0.020 kg/ha) at 360 DAA. This information may aid in the development of more effective management of alligatorweed by herbicide application.