Tillage operations significantly influenced the vertical distribution of winter weeds in the wheat field. Significantly higher number of seeds ofAvena ludoviciana, Medicago hispida and Chenopodium album was recorded in the upper 0-5 cm layer under zero tillage treatments than conventional and deep tillage. But athigher depths of 5-10 and, 10-15 cm, the number of seeds of A. ludoviciana was significantly higher under deep tillage followed by conventional and zero tillage. Continuous use ofclodinafop fb 2, 4-D and isoproturon+2, 4-D for control of weeds in wheat field significantly reduced the number of weed seeds over weedy check.