Surface active formulation from non edible oil was evaluated for herbicidal activity against weeds, Checorium intybus and Medicago denticulata under in vitro conditions.  Different dilutions of formulation exhibited markedly variable herbicidal activities against germination and seedling growth of the target weed species. Different concentrations (1.0 – 7.5%)  inhibited the growth of shoot and root of C. intybus and M.denticulata  by 71.7 – 100, 83.3 – 97.7% and 51.17 – 100, 24 – 100%, respectively. No shoot formation was recorded in 5.0 and 7.5% dilution against C. intybus and  M. denticulate. The present study concluded that the surfactant formulations from non edible oil of Pongamia pinnata possess potential herbicidal activities against weeds. Pollution of the environment is now a major concern. Therefore, utilization of plant product based surfactant as herbicide will pave a way for possible exploitation of surfactants in weed management.