A field experiment was conducted during the Rabi season of 2008-09 and 2009-10 on sandy loam soils of research farm of Regional Station, Bathinda to find out response of different varieties of barley and various combinations of herbicides on growth, weed dry matter and grain yield of barley under semi arid conditions of Punjab. The experiment was laid out in split plot design with two varieties in main plots and 12 different herbicide combinations in sub plots. The grain yield had no significant difference with respect to two varieties but it varied significantly with various combinations of herbicides. The maximum mean grain yield i.e. 3.46 t/ha was recorded in pinoxaden + metsulfuron 50 + 4 g/ha (T4) followed by metsulfuron fb pinoxaden 4 + 50 g/ha, metsulfuron fb pinoxaden 4 + 45 g/ha and lowest (2.31 t/ha) in control. The dry weight of weed flora was significantly higher in control plots as compared to other treatments during both the years and no significant difference was observed in both the varieties with respect to dry weight of weed flora.